For any particular experiment prepare a smear culture on agar and incubate at 37° C. for 24 hours anaerobically.
anaerobic an·aer·o·bic (ān'ə-rō'bĭk, -â-rō'bĭk)
Relating to or being an anaerobe.
Living without oxygen.
Occurring in the absence of oxygen or not requiring oxygen to live. Anaerobic bacteria produce energy from food molecules without the presence of oxygen. Compare aerobic.