The cover glass being in position, the tube is closed by screwing on the cap.
Put a droplet of this blood on a glass slide, cover with cover glass and examine with high power of the microscope.
Allow a drop of dilute acetic acid to run under the cover glass; the filaments will swell and become transparent.
If air bubbles interfere with the examination, they may be driven off with alcohol, and then the cover glass put on.
Cover with the cover glass and examine under the microscope.
The potash should now be removed as completely as possible with blotting paper, and pure water run under the cover glass.
Over the fibre was a cover glass with a small scale marked on it.
If the preparation is to be kept permanently, the acid must all be washed out, and dilute glycerine run under the cover glass.
Place a drop of milk on a microscopical slide and cover with cover glass.
By light pressure upon the cover glass the bacilli are easily pressed out of the masses of detritus and secretion.