Take up the required dose of inoculum into a sterilised pipette.
Charge a platinum loop with a small quantity of the inoculum.
Flame the plugs, open the tubes, sterilise the platinum needle and charge it with the inoculum as in the previous cultivations.
Add one loopful of the inoculum to tube No. 1, treating the liquefied medium as bouillon.
Thrust the needle of the injecting syringe boldly into the muscular tissue and inject the inoculum slowly.
Introduce the inoculum in the form of an emulsion by means of another pipette.
Attach one needle to the syringe and take up the required dose of inoculum and remove the needle.
Should any drops of the inoculum be forced out, they will fall on the filter paper, which should be immediately burned.
Direct the assistant to cease compressing the root of the ear, and slowly inject the inoculum.
inoculum in·oc·u·lum (ĭ-nŏk'yə-ləm)
n. pl. in·oc·u·lums or in·oc·u·la (-lə)
The microorganisms or other material used in an inoculation. Also called inoculant.