Some forms, ordinarily producing light, will grow, but fail to luminesce at high temperatures.
Luminous bacteria are also very sensitive to oxygen and cease to luminesce in its absence.
Esculin will not luminesce with peroxidase and H2O2, but pyrogallol or gallic acid will.
On the other hand, a great many forms are able to luminesce quite independently of previous illumination.
Luminous bacteria will continue to luminesce although they are grown in the dark for many weeks.
This means that for a definite temperature, say, 20°, phosphorus will not luminesce with an oxygen pressure of 583 mm.
They only luminesce at night and fail to respond to stimulation or are difficult to stimulate during the day.
In the case of some of these oxidizers pyrogallol will luminesce in dilute concentrations but not in strong.
Also, dilute pyrogallol will luminesce with a dilute solution of oxidizer but not with a concentrated solution of oxidizer.
Phosphorus will only begin to luminesce at a certain small pressure of oxygen.