blot analysis

noun

1. Also called: blotting. biochem a technique for analysing biological molecules, such as proteins ( Western blot analysis ), DNA ( Southern blot analysis ), and RNA ( Northern blot analysis ), involving their separation by gel electrophoresis, transfer to a nitrocellulose sheet, and subsequent analysis by autoradiography

Example Sentences

By using a technique called western blot analysis — separating and identifying all the proteins in a tissue sample — the authors found MMP-2 cleaves off a portion of the leptin receptor in the hypothalamus, impairing the hormone’s signaling and its ability to suppress appetite.

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By using a technique called western blot analysis—separating and identifying all the proteins in a tissue sample—the authors found MMP-2 cleaves off a portion of the leptin receptor in the hypothalamus, impairing the hormone’s signaling and its ability to suppress appetite.

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J.A.S. and K.T.B. performed western blot analysis.

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Scale bar 30 μm. e, Western blot analysis of sub-fractionated control MCF7-RAS cells and MCF7-RAS–Tet/ON-LACTB-expressing cells with 24 h of DOX treatment.

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Scale bar 30 μm. e, Western blot analysis of sub-fractionated control MCF7-RAS cells and MCF7-RAS–Tet/ON-LACTB-expressing cells with 24 h of DOX treatment.

Read more on Nature