restriction enzymes
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Standard methods for assembling long DNA molecules typically use restriction enzymes to make cuts and T4 DNA ligase to connect the resulting fragments.
From Science Daily ● Aug. 19, 2026
Developed by synthetic biologist Sylvestre Marillonnet and his colleagues at Icon Genetics in Halle, Germany, Golden Gate uses specialized proteins known as type IIS restriction enzymes to make targeted cuts in DNA strands4.
From Nature ● Feb. 23, 2020
Instead of reproducing DNA replication in a test tube and making different fragment lengths with special nucleotides, a DNA sample is chopped into pieces with special molecular scissors called restriction enzymes.
From Slate ● Feb. 5, 2019
These plasmid vectors contain many short DNA sequences that can be cut with different commonly available restriction enzymes.
From Textbooks ● Apr. 25, 2013
For example, basic studies of bacterial resistance to viruses led to the discovery of restriction enzymes, the biological scissors that can snip DNA segments at precisely defined locations.
From Time Magazine Archive
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