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had copulated

  • past perfect
    of copulate.
    copulate
    verb (used without object)
    to engage in sexual intercourse.

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On the seventeenth we found no eggs in the hive; the queen was as slender as the first day; therefore the male, with which she had copulated, had not impregnated her eggs.

From New observations on the natural history of bees by François Huber

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