Close the tubulure by means of an india-rubber stopper previously sterilised by boiling in a beaker of water.
The tubulure and the side tubes are plugged with cotton-wool.
Remove the cotton-wool plug from the tubulure and pour the inoculated medium into the glass vessel.
B is tubulure for filling the apparatus, and is stopped with a cap.
The steam enters through the tubulure, A, and finds its way around the periphery of a tuyere, D.
This tubulure permits of emptying the boiler into the reservoir, L.
Twenty pounds of strong sulphuric acid are now to be introduced by the tubulure c, and then its lid must be put on.