Gram's method

American  

[gramz] / græmz /

noun

(sometimes lowercase)

1. a method of staining and distinguishing bacteria, in which a fixed bacterial smear is stained with crystal violet, treated with Gram's solution, decolorized with alcohol, counterstained with safranine, and washed with water.

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Gram's method British  

noun

1. bacteriol a staining technique used to classify bacteria, based on their ability to retain or lose a violet colour, produced by crystal violet and iodine, after treatment with a decolorizing agent See also Gram-negative Gram-positive

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Etymology

Origin of Gram's method

Named after Hans C. J. Gram (1853–1938), Danish bacteriologist

Example Sentences

See Examples For:

Gram's method

From the resulting growths make cover-slip preparations and stain carbolic methylene-blue, Neisser's method, Gram's method.

From The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. by Eyre, J. W. H. (John William Henry)

In Gram's method, however, only some bacteria retain the stain, while others lose it.

From Encyclopaedia Britannica, 11th Edition, Volume 3, Part 1, Slice 2 "Baconthorpe" to "Bankruptcy" by Various

Prepare cover-slip preparations, fix and clear as for milk preparations, stain carbolic methylene-blue, Gram's method, Ziehl-Neelsen's method, and examine microscopically with a 1/12 inch oil-immersion lens.

From The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. by Eyre, J. W. H. (John William Henry)

Glanders is due to the action of a specific bacterium, the bacillus mallei, which resembles the tubercle bacillus, save that it is somewhat shorter and broader, and does not stain by Gram's method.

From Manual of Surgery Volume First: General Surgery. Sixth Edition. by Thomson, Alexis

It stains with moderate rapidity with the ordinary anilin dyes, and is decolorized by Gram's method.

From Contagious Abortion of Cows by MacNeal, Ward J.