endonuclease

American  

[en-doh-noo-klee-eys, -eyz, -nyoo-] / ˌɛn doʊˈnu kliˌeɪs, -ˌeɪz, -ˈnju- /

noun

Biochemistry.

1. any of a group of enzymes that degrade DNA or RNA molecules by breaking linkages within the polynucleotide chains.

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endonuclease British  

/ ˌɛndəʊˈnjuːklɪˌeɪz /

noun

1. an enzyme that is responsible for scission of a nucleic acid chain, the action of which is not confined to the terminal nucleotide Compare exonuclease

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Etymology

Origin of endonuclease

First recorded in 1960–65; endo- + nuclease

Example Sentences

See Examples For:

endonuclease

The paper is titled "Activation of CBASS-Cap5 endonuclease immune effector by cyclic nucleotides."

From Science Daily ● Feb. 6, 2024

But the challenge was how to introduce the endonuclease gene and its associated genetic payload to a spot in the genome where it would work safely without inadvertently affecting other aspects of the animal’s physiology.

From Scientific American ● Jan. 13, 2023

Researchers need only an enzyme that cuts DNA, such as the endonuclease Cas9, and a guide RNA molecule that directs the enzyme to cut the gene of interest.

From Nature ● Sep. 26, 2017

The F1 were genotyped using fin clipping, DNA isolation, PCR, T7 endonuclease to identify heterozygous mutant fish followed by cloning and sequencing of the mutant PCR products to validate presence of the frameshift allele.

From Nature ● Dec. 13, 2016

Mosaic mutant F0 fish were outcrossed to AB wild-type fish and embryos were batch genotyped for transmission of the mutation using PCR and T7 endonuclease.

From Nature ● Dec. 13, 2016